c5cfac679b Samples treated with the Bradford assay. Example[edit]. Data for known concentrations of protein are used to make the standard curve, plotting concentration on the X axis, and the assay measurement on the Y axis. To analyze the data, one locates the measurement on the Y-axis that corresponds to the assay measurement of the unknown substance and follows a line to intersect the standard curve. The corresponding value on the X-axis is the concentration of substance in the unknown sample. Bradford Assay Background . Some suggestions: Go back to the last page Go to the home page .. Your browser doesn't accept cookies. Multiple samples with known properties are measured and graphed, which then allows the same properties to be determined for unknown samples by interpolation on the graph. The concentration of protein in a different sample is determined by determining where on the standard curve it should go - in this case, 30 milligrams.
The reagent Coomassie Brilliant Blue turns blue when it binds to arginine and aromatic amino acids present in proteins, thus increasing the absorbance of the sample. (pdf format) "Bradford Protein Assay" (PDF). Retrieved May 31, 2005.. Cookies are required to use this site. In this case, the greater the absorbance, the higher the protein concentration. See also[edit]. Color Linear regression Logarithmic scale Protein Serial dilution . 403 Forbidden . Bio-Rad Quick Starttm Bradford Protein Assay Instructional Manual.
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